The inputs are (multi-)fasta format sequences.The LAST download includes some in its "examples" directory. Download from the page http://last.cbrc.jp/archive/ Adaptive seeds tame genomic sequence comparison. SM Kielbasa, R Wan, K Sato, P Horton, MC Frith, Genome Research 2011. | Incorporating sequence quality data into alignment improves DNA read mapping. MC Frith, R Wan, P Horton, NAR 2010. | Parameters for accurate genome alignment. MC Frith, M Hamada, P Horton, BMC Bioinformatics 2010. LAST is a software for comparing and aligning sequences, typically DNA or protein sequences. LAST is similar to BLAST, but it copes better with very large amounts of sequence data. It can also report probabilities for every pair of aligned letters, indicating the reliability of each pairing.