More information XML output
| Name | LfDB |
|---|---|
| Aliases | Lectin Frontier DataBase |
| Description | Lectin Frontier DataBase (LfDB) provides quantitative interaction data in terms of the affinity constants (Ka) of a series of lectins toward a panel of pyridylaminated (PA) glycans obtained by an automated frontal affinity chromatography with fluorescence detection (FAC-FD) system (Fig. 1). Since the data are accurate and reliable, providing the absolute values of sugar-protein interactions, LfDB would be a valuable resource in the studies of glycan-related biology.(cited from the original site) |
| Type | DB |
| Main Institutes of management | Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST) |
| Country of the Institute | Japan |
| URL of the site | http://riodb.ibase.aist.go.jp/rcmg/glycodb/LectinSearch |
| Interface | GUI |
| Input example | 1: Type keyword or choose categories among Lectin family, Monosaccharide Specificity, or 3D-fold. Click Show All to view the full lectin list. (cited from the original site) |
| Keyword |
An automated frontal affinity chromatography with fluorescence detection | Lectin | pyridylaminated glycans
|
| Amount of the all data for download（Mbyte） | Method to obtain the all data. | - | None |
| External resources (databases) in building the product. | None |
| Data type | Sugar chain ,Lectin |
| Biological species in the main concern |
All species
|
| Conditions of use | Under investigation |
| Frequency of updates (in last two years) | 1 |
| Last date of updates (date of confirmation) | 0000/00/00 (2009/02/04) |
| Main IDs used in the products | None |
| How to make a link to get access to each IDs. | None |
| external databases to which this database/tool have links | NCBI (http://www.ncbi.nlm.nih.gov) | Pfam (http://pfam.janelia.org) | PDB (http://www.rcsb.org/pdb/home/home.do) |
| Published papers (PubMed IDs) | [1] Kasai K, Ishii S. Quantitative analysis of affinity chromatography of trypsin. A new technique for investigation of protein-ligand interaction. J Biochem 1975; 77:261-4. [2] Hirabayashi J, et al. Oligosaccharide specificity of galectins: a search by frontal affinity chromatography. Biochim Biophys Acta 2002; 1572:232-54. [3] Hirabayashi J, et al. Frontal affinity chromatography as a tool for elucidation of sugar recognition properties of lectins. Methods Enzymol 2003; 362:353-68. [4] Nakamura-Tsuruta S, et al. Comparative analysis of carbohydrate-binding properties of two tandem repeat-type Jacalin-related lectins, Castanea crenata agglutinin and Cycas revoluta leaf lectin.. FEBS J 2005; 272:2784-99. [5] Tateno H, et al. Frontal affinity chromatography: sugar-protein interactions. Nat Protoc 2007; 2:2529-37. (cited from the original site) |